DSIP 5 mg
Delta Sleep-Inducing Peptide · nonapeptide of 9 amino acids
In stock · ships within 24 hResearch areas
- Delta sleep
- Stress response
- ACTH and cortisol
- Hypoxia
- Circadian rhythm
Topics of published studies (cell, animal and clinical). These are not claims about effects in humans; the product is intended solely for research. You will find the sources below.
The story of the sleep peptide- Content
- 5 mg
- Length
- 9 amino acids
- Mol. weight
- 848.8
- CAS
- 62568-57-4
Z-Box, pick-up point or home delivery- Ships from the Czech Republic within 24 hours
- Free shipping over CZK 3,000
- Discreet packaging
Main research areas
- Sleep: Studied for the share of deep (delta) sleep on EEG and for sleep quality.
- Stress axis: In studies it reduced the release of ACTH and cortisol.
- Hypoxia: In animals it protected cells during oxygen deprivation.
- Neuromodulation: Its relationship to opioid and GABA signalling is being studied.
Preparing a stock solution
Preparing a stock solution
The vial contains 5 mg. Dissolving it in 3.0 ml of solvent gives a stock solution with a concentration of 1.67 mg/ml, i.e. 1.67 µg in every microlitre. In the laboratory the solvent used is sterile or bacteriostatic water, or a buffer according to the method. Add the solvent slowly down the inside wall of the vial and let the contents dissolve with gentle swirling, not shaking – shaking can damage the peptide. Divide the stock solution into aliquots and freeze them; repeated thawing reduces the content. The reconstitution calculator works out conversions for other volumes and dilutions using C1V1 = C2V2.
Limitations and research notes
- DSIP is not a registered medicinal product.
- Published work comes mainly from older studies.
- The peptide has low stability in solution; use it within weeks.
In 1977, Swiss scientists transferred blood from sleeping rabbits into the brains of awake animals – and those animals began to fall into deep sleep. From the blood they then isolated the substance responsible: a nine-amino-acid peptide they named DSIP (Delta Sleep-Inducing Peptide).[1]
What delta sleep is
Sleep runs in cycles: light sleep, deep sleep and REM sleep repeat several times a night. The deepest stage is called delta sleep (N3), after the slow delta waves on the EEG. This is when the body regenerates most and releases the most growth hormone. Delta sleep is most abundant in the first half of the night and declines with age – the graphic above shows the course of a night schematically.
DSIP peptide and sleep in studies
The discovery was followed by a wave of research. A review by Graf & Kastin (1984) concluded that the peptide's effects on sleep were observed in many studies but were not always the same – they depended on dose, timing and animal species.[2] Small studies in people with insomnia described improved sleep quality, while others found no marked effect. The peptide thus came to be seen as a sleep regulator rather than a “sleeping pill”.
Stress, cortisol and cell protection
Other effects gradually proved more interesting. In studies it influenced the stress axis – reducing the release of ACTH and cortisol – dampened the stress response and, in animals, protected cells during oxygen deprivation (hypoxia).[2][3] That is why today it is studied mainly in models of the stress response and sleep.
| Area | What studies look at |
|---|---|
| Sleep | Share of delta sleep on EEG, sleep quality |
| Stress axis | ACTH and cortisol levels |
| Hypoxia | Cell protection during oxygen deprivation |
| Pain and withdrawal | Older clinical work on withdrawal symptoms |
Sleep and recovery
Why is deep sleep so important? During it the brain processes the day's information, stress hormone levels fall and growth hormone, which drives tissue repair, peaks in the blood. Short or interrupted deep sleep is therefore linked to poorer recovery, fatigue and higher cortisol. Research into substances that affect deep sleep thus goes beyond insomnia itself – it also concerns recovery after exertion and coping with long-term stress.
An unsolved mystery
Even after decades it is not clear exactly how it works. Scientists have still not found its gene or its own receptor – which is why the review by Kovalzon & Strekalova (2006) is titled “a still unresolved riddle”.[3] That is exactly what makes it an interesting research tool. You will find details in the article DSIP: the delta sleep-inducing peptide and what we know about it.
DSIP 5 mg: preparation and availability
The vial contains 5 mg of peptide. Adding 3.0 ml of bacteriostatic water gives a solution of about 1.67 mg/ml; you will find the conditions used in published studies in the Solution preparation and published studies section, and the reconstitution calculator does the conversions. For stress research we also offer Selank.
This is not an approved medicine. The product is intended solely for research.
Questions about DSIP
What is DSIP?
Delta Sleep-Inducing Peptide – the nonapeptide Trp-Ala-Gly-Gly-Asp-Ala-Ser-Gly-Glu, isolated in 1977 from the blood of sleeping rabbits. Transferring it into the brains of awake animals induced deep sleep.
Does DSIP help with sleep?
Studies describe effects on the share of delta sleep and on sleep quality, but the results were not consistent. Today DSIP is seen more as a regulator of sleep and stress than as a sleeping pill.
How does DSIP affect stress?
In studies it reduced the release of ACTH and cortisol and dampened the stress response. In animals it also protected cells during oxygen deprivation.
Why is DSIP an “unsolved mystery”?
Neither its gene nor its own receptor has been found to this day, so it is not clear exactly how it is produced and how it works in the body.
What DSIP doses were used in studies?
Most studies date from the 1970s and 1980s and worked with animals – DSIP was given into the brain ventricles or intravenously. You will find an overview with sources in the Preparation and doses in studies section.
We will publish the DSIP certificate of analysis with the first batch
We send every new batch for independent testing. As soon as DSIP 5 mg is in stock, you will find the lab report here with the purity, identity and vial content results – just like for our other products on the peptide test results page.
Laboratories we work with: Janoshik Analytical, Finnrick and LiquiLabs.
- Purity (HPLC)Share of DSIP in the powderawaiting batch
- Identity (MS)Mass 848.8 g/molawaiting batch
- Vial contentDeclared 5 mgawaiting batch
Molecular profile – sequence
Trp-Ala-Gly-Gly-Asp-Ala-Ser-Gly-Glu
Molecular weight
- 848.82 g/mol
CAS number
- 62568-57-4
Molecular formula
- C35H48N10O15
Material data
Appearance
White to off-white lyophilized powder
Solubility
Soluble in bacteriostatic water and suitable aqueous laboratory solvents.
Product note: Delta sleep-inducing peptide (nonapeptide); supplied solely for research purposes.
Peptides in transit
We ship lyophilized peptides in glass vials by standard shipping; they do not need refrigeration. Short temperature fluctuations in transit do not reduce their quality, and the lyophilized form stays stable for several weeks even in summer temperatures.
Storing lyophilized peptides
Store lyophilized peptides in a dry place, protected from light and moisture. We generally recommend these conditions:
- Room temperature (15–25 °C): up to 3 months
- In the fridge (2–8 °C): up to 6 months
- In the freezer (−20 °C and below): 3–5 years (recommended for long-term storage)
After taking the vial out of the fridge or freezer, let it warm to room temperature.
Storing reconstituted peptides
Peptide solutions are generally less stable after reconstitution than the lyophilized form, so keep them cold whenever possible. We generally recommend these conditions:
- Room temperature (15–25 °C): not recommended long term
- In the fridge (2–8 °C): usually 4–8 weeks
- In the freezer (−20 °C and below): up to 3–4 months
To keep the peptide intact, avoid repeated freezing and thawing, which can speed up its degradation.
Important:
Most manufacturers recommend using reconstituted peptides stored at 2–8 °C within 4 weeks. This recommendation is deliberately cautious and may not reflect the actual chemical stability of every peptide. Actual stability depends strongly on the sequence and formulation of the specific peptide, so stick to the recommended period and keep the solution cold and dark.
- Schoenenberger GA, Monnier M. Characterization of a delta-electroencephalogram (-sleep)-inducing peptide. Proceedings of the National Academy of Sciences USA. 1977;74(3):1282–1286. Find on PubMed ↗
- Graf MV, Kastin AJ. Delta-sleep-inducing peptide (DSIP): a review. Neuroscience & Biobehavioral Reviews. 1984;8(1):83–93. Find on PubMed ↗
- Kovalzon VM, Strekalova TV. Delta sleep-inducing peptide (DSIP): a still unresolved riddle. Journal of Neurochemistry. 2006;97(2):303–309. Find on PubMed ↗
The research overview is based on scientific literature
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DSIP 5 mg is intended solely for in vitro research and laboratory use. The information on this page summarises published studies and is not medical advice.
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